Figure 4
From: TDP-43 self-interaction is modulated by redox-active compounds Auranofin, Chelerythrine and Riluzole
Flowchart of NanoBit luciferase measurement for verification dose-response analysis. Description of measurement procedure for selected compounds from LOPAC®1280 library. For reduction of variability, luciferase reading from each well after treatment has been normalized to the same well before treatment (TDP-43 ratio). To detect effects on cell viability, TDP-43 ratio has been calculated in relation to cell viability measurement via WST-8 assay in the same well (TDP-43 ratio-tox). For avoidance of off-target effects of compounds such as interference with luciferase reaction, protein homeostasis and TK promoter activity, we applied the same compounds onto N2a cells transfected with a TK-promoter construct expressing full length NanoLuc luciferase. Therefore, results of TDP-43 self-interaction were normalized with respect to NanoLuc activity (Normalized TDP-43).
