Figure 1
From: The FGFR4-388arg Variant Promotes Lung Cancer Progression by N-Cadherin Induction
Effect of the overexpression of the 388Gly and 388Arg variants of FGFR4 in the tumorigenic abilities of the inmortalized NL20, the lung SCC H226 and Calu-1, and the H2009 and HCC827 lung ADC cell lines. (A) 10% FBS growth curves. (B) soft agar assays showing the relative colony number quantification (left) and representative images (right). (C) Western blot determination of activation of cancer-related signalling pathways of 388Gly and 388Arg FGFR4-overexpressing cell lines. In soft agar assay, colony number representation is shown. All values were normalized to empty vector control for each replicate of the experiment, and the mean and standard deviation of every normalized replicate are represented. For western blotting, cells were serum starved for 5 hours and then the protein extraction was made. For the serum stimulated conditions, after serum starvation cells were stimulated with serum-containing complete medium for fifteen minutes before protein extraction. p-Values are represented as asterisks (*p < 0,05; **p < 0,01; ***p < 0,001). EV = Empty Vector control, FGFR4-Gly = FGFR4-388Gly overexpressing, FGFR4-Arg = FGFR4-388Arg overexpressing, FBS = Fetal Bovine Serum.
