Figure 3
From: The FGFR4-388arg Variant Promotes Lung Cancer Progression by N-Cadherin Induction
Overexpression of the 388Arg variant of FGFR4 induces the expression of EMT markers in lung cell lines. The mRNA (A) and protein (B) expression of four EMT markers (N-cadherin, vimentin, Twist1 and Snail1) was performed in the control empty vector (EV), FGFR4-388Gly-overexpressing (FGFR4-Gly) and FGFR4-388Arg-overexpressing (FGFR4-Arg) lung immortalized NL20 cell line and lung SCC H226 and Calu1, and ADC H2009 and HCC827 cell lines. (C) Relative number of migrated cells of the previously mentioned cell lines. The mRNA measurements were performed in three independent experiments and mean expression values, represented as 2−ΔCt with their respective standard deviation, are represented. For western blots, a representative blot is shown. Migration assays were performed in three independent experiments. All values were normalized to empty vector control for each replicate of the experiment and the mean and standard deviation of every normalized replicate are represented. p-values are represented as asterisks (*p < 0,05; **p < 0,01; ***p < 0,001, ns = non significant). EV = Empty Vector control, FGFR4-Gly = FGFR4-388Gly overexpressing, FGFR4-Arg = FGFR4-388Arg overexpressing.
