Table 1 Sequence of primer used.
S.NO | Primer | Primer Sequence | Accession Number |
|---|---|---|---|
1 | β-Actin | F: 5′-CGAGCAGGAGATGGGAACC-3′ R: 5′-CAACGGAAACGCTCATTGC-3′ | AF057040 |
2. | iNOS | F: 5′-GGAGATGCAAGGTCAGCTTC-3′ R: 5′-GGCAAAGCTCAGTGACTTCC-3′ | AY324390 |
3 | TNF-α | F: 5′-ACCAGGCCTTTTCTTCAGGT-3′ R: 5′-TGCCCAGTCTGTCTCCTTCT-3′ | AY427649 |
4 | IL-1β | F: 5′-ATCCAAACGGATACGACCAG-3′ R: 5′-TCGGTGTCTTTCCTGTCCAT-3′ | BC098597 |
5. | IL-4 | F: 5′-AGTCACGCTGCTGATGAAGA-3′ R: 5′-AACTTGGTCTTGGGCTTTTT-3′ | AB375404 |
6. | IL-10 | F: 5′-ATAGGATGTTGCTGGGTTGG-3′ R: 5′-GTGGATGAAGTCCATTTGTGC-3′ | AY887900 |
7. | TLR-4a | F: 5′-CGGCACTCCTCAAATCAACT-3′ R: 5′-GTCCTTCAAATCCTCCCACA-3′ | NM _001131051 |
8. | IFN-γ | F: 5′-ATGATTGCGCAACACATGAT-3′ R: 5′-ATCTTTCAGGATTCGCAGGA-3′ | AB158361 |
