Figure 2 | Scientific Reports

Figure 2

From: Temporal reprogramming of calcium signalling via crosstalk of gonadotrophin receptors that associate as functionally asymmetric heteromers

Figure 2

Sustained LHR/FSHR Ca2 + release is Gαq/11 and Gβγ-dependent. (a,b) HEK 293 cells stably expressing LHR were pre-treated with FR900359, a Gαq/11 inhibitor (a) (1 μM, 2 h) or a Gβγ inhibitor gallein (b) (1 h) and intracellular Ca2+ levels in response to LH (100 nM) were measured. Representative fluorescent traces over time with baseline fluorescence subtracted are shown in a(i) and b(i). For a(ii) and b(ii) results are expressed as area under the curve from n = 3–5 experiments. **p < 0.01, ****p < 0.0001.

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