Figure 2
From: OPA1 gene therapy prevents retinal ganglion cell loss in a Dominant Optic Atrophy mouse model
Assessment of the vector transduction efficiency. (A) Quantification of murine endogenous Opa1 transcripts as well as GFP transcripts. MmOpa1: Mus musculus Opa1 (B) In vivo funduscopy was performed on untreated and treated Opa1+/− mice in brightfield (upper panel) and using a specific green filter to observe GFP fluorescence (lower panel). (C) Post-mortem immunohistochemistry was done on whole mount retinas using a RGC specific Brn3a labelling (Upper panel). Zoom-in (lower panel) was done to observe Brn3a/GFP double-positive RGCs (double arrow), Brn3a positive (arrow) and GFP negative RGCs.
