Figure 4
From: Efficient non-cytotoxic fluorescent staining of halophiles
Conversion of Hbt. salinarum cells stained with MitoTracker Orange CMTMRos into spheroplasts by EDTA treatment. The entire process lasts approximately 10 min: in the beginning the cells have a clear rod shape (A; t = 0 s). During the conversion, the short cells swell at one end (B, arrows; t = 200 s), while long cells first bend around their midpoint (C, arrows; t = 260 s). Finally, both types of cells form spheroplasts (D, t = 480 s). Cells with intermediate shapes (swollen or bent) are indicated with arrows.
