Figure 6
From: Efficient non-cytotoxic fluorescent staining of halophiles
Bleaching of stained giant rod Hbt. salinarum cells. The cytosol integrity of the cells (approximately 40 µm long in this case) was demonstrated using FLIP of MitoTracker Orange CMTMRos in Hbt. salinarum cells. The bleaching region is marked with a white square, the intensity of the fluorescence signal is shown as a heatmap (pseudocolour, intensity increases from purple to red according to the scale below image). The loss of fluorescence intensity along the entire length of the cell shows that the majority of the dye can freely diffuse along the cell, demonstrating the existence of one cellular compartment not separated by septal membranes.
