Figure 1
From: Intracellular tracing of amyloid vaccines through direct fluorescent labelling
Representative fluorescence microscopy of whole THP-1 cells cultured for 1 h in R10 medium containing 10 μM thioflavin T (ThT) in the presence of 4 μM Aβ42. Cells were fixed in 4% w/v PFA, washed in 50 mM PIPES buffer, pH 7.4 before mounting with Fluoromount™ (Sigma Aldrich) on poly-lysine coated slides. ThT fluorescence (green) is depicted (Olympus long bandpass U-WMBV2 filter cube) with the magnified insert denoted (*) of which the bright-field image has been overlaid. White arrows highlight the presence of ThT-reactive deposits within THP-1 cells. Magnification X 1000, scale bar: 20 μm.
