Figure 4
From: Fibroblast-associated tumour microenvironment induces vascular structure-networked tumouroid
Change in gene expression by fibroblasts during tumour progression. (a) Experimental scheme for gene expression profile. Indicated cells were cultured in 2D and in the 3D microfluidic device, and RNA was extracted from cells at the indicated time points. (b) RNA was used for the microarray to establish the gene expression profile. The Venn diagram presents genes expressed differentially between groups of arrays; the expression of 1350 genes was visualized using Heatmap software. (c) Total of 1350 genes was analyzed based on Ingenuity Pathway Analysis (IPA), and the signaling pathway was categorized according to IPA results. (d) Expression levels of genes involved in metastasis, apoptosis, and angiogenesis are shown. (e,f) For gene networks, VEGF and PTEN-enriched signaling pathways were visualized based on IPA analysis. Abbreviations: ATP2C1; Calcium-transporting ATPase type 2C member 1, IL6; Interleukin-6, VCAN; Versican, VEGFB; Vascular endothelial growth factor beta, ERBB3; Erb-B2 receptor tyrosine kinase 3, CDCA2; Cell division cycle-associated protein 2, TP53I3; Tumour protein p53 inducible protein 3, TGFBI; Transforming growth factor beta induced, RASSF1; Ras Association Domain Family Member 1, PTEN; Phosphatase and tensin homolog, AGT; Angiotensinogen, ADGRG1; Adhesion G protein-coupled receptor G1, CXCL2; Chemokine ligand 2, RSPO3; R-spondin 3, IL1A; Interleukin 1 alpha.
