Figure 1 | Scientific Reports

Figure 1

From: High dietary salt intake correlates with modulated Th17-Treg cell balance resulting in enhanced bone loss and impaired bone-microarchitecture in male mice

Figure 1

High dietary salt intake enhances bone loss. (A) Experimental work plan. Mice were divided into 3 groups. The high salt diet group (HSD) mice received diet containing 4% NaCl in chow plus 1% in drinking water. The low salt diet group (LSD) mice were fed on diet having 0.4% NaCl in chow plus normal drinking water whereas normal mice were given a diet containing 0.8% NaCl in chow plus normal drinking water for 45 days. At the end of experiment mice were sacrificed and tissues (bones+ lymphoid) were analysed. (B) 2D SEM images of femur cortical. (C) 2D MATLAB analysis of SEM images. (D) 3D AFM images of femur cortical. (E) 3D MATLAB analysis of AFM images. The above images are representative of one experiment and similar results were obtained in three different experiments with n = 10 mice/group/experiment. (Mouse image courtesy: Hamid Y. Dar).

Back to article page