Figure 2
Skeletal muscle integrity and mitochondrial biogenesis in male TG/Nrf2-Ko mice. (A) Grip strength of 24 wks old animals (n = 8–10). (B) 24 h voluntary running wheel activity of 14 wks old male mice (n = 9–10). (C) Skeletal muscle mRNA expression in quadriceps muscle of the myosin heavy chain (Mhc) fiber type markers and (D) mitochondrial biogenesis markers of 24 wks old animals (n = 8–10). (E) Representative immunoblots of PGC1α/β in quadriceps muscle from 24 wks old mice from cropped blots of the same gel using αTubulin (αTUB) as loading control (2 samples shown out of 4–5 analyzed per group). (F) Representative immunoblots of phospho-AMPKThr172 and total AMPK in quadriceps muscle from 24 wks old mice from different gels processed in parallel (2 samples shown out of 4–5 analyzed per group). (G) Skeletal muscle (gastrocnemius) citrate synthase activity of 24 wks old animals (n = 4–8). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; **p < 0.01; ***p < 0.001.
