Figure 1 | Scientific Reports

Figure 1

From: CD63, MHC class 1, and CD47 identify subsets of extracellular vesicles containing distinct populations of noncoding RNAs

Figure 1

Characterization of Jurkat T cell EV fractions and CD47 expression. (a,b) EVs were extracted from wild type (a) and CD47-deficient Jurkat T cells (b) using the Exo-Quick kit, and vesicle size and concentration were quantified by Nanosight analysis. (c) EVs released by Jurkat cells were labeled using Bodipy-FL and captured with anti-CD63-MNPs (upper panel) or with anti-MHC I-MNPs (lower panel) and stained with PE-conjugated anti-CD47 or isotype control antibodies. Representative experiment out of 3. (d) EVs released by CD47-deficient JinB8 cells were captured with anti-CD63-MNPs (upper panel) or with anti-MHC I-MNPs (lower panel) and stained with anti-CD47 or with isotype control antibodies. Representative experiment of out of 3. (e) Size distribution of CD47+ EVs captured with anti-CD63-MNPs (red bars) or with anti-MHC1-MNPs (black bars). Representative experiment out of 3. (f) EVs released by Jurkat cells were captured with anti-CD47-MNPs and stained for CD63 antigen. Volumetric control was used to estimate concentration of CD47+CD63+ EVs. One representative experiment is presented out of 2 performed.

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