Figure 3

Enrichment of small RNAs in antibody captured EVs. (a) Scatter graph of data from 3 biological replicates comparing RNA abundance in CD47⁺ and CD47⁻ EVs. A total of 680 transcripts were significantly enriched or depleted in captured EVs (CD47_CAP) versus uncaptured EVs (CD47_UnCap, p < 0.05). Data are presented as log base 2 of total RPM. White dots indicate >8-fold enrichment or depletion. Red and blue indicate >2-fold enrichment or depletion, respectively. (b) Scatter graph of data from 2 biological replicates comparing RNA abundance in CD63⁺ and CD63⁻ EVs. A total of 1339 transcripts were significantly enriched or depleted in captured EVs (CD63_CAP) versus uncaptured EVs (CD63_UnCap). (c) Scatter graphs of data from 2 biological replicates comparing RNA abundance in MHC1⁺ and MHC1⁻ EVs. A total of 782 were significantly enriched or depleted in captured EVs (MHC1_CAP) versus uncaptured EVs (MHC1_UnCap). (d–e) Graphs presenting the numbers of significantly enriched or depleted RNAs belonging to the indicated classes comparing captured versus uncaptured CD47⁺ CD63⁺ and MHC1⁺ EV samples.