Figure 2
Soluble factor PGE2 secreted from Hp treated GSCs. (a) Plating 2 × 105 GSCs cells/well with or without Hp infection (MOI = 1:200) for 24 hrs. (b) Using transwell co-culture experiment, plating 2 × 105 GSCs cells/well with Hp infection (MOI = 1:200) in the apical side of transwell insert with a total of 2 × 105 DCs were cultured in basolateral side of 24-well plates for 24 hrs. Supernatants were collected for PGE2 analysis by ELISA. GSCs N1 derived from a Hp negative, non-cancer control subject was used for reference. Paired GSCs of tumor (T2, T6, T9, and T21) and adjacent normal part (N2, N6, N9, N21) were obtained from gastric cancer patients. PGE2 data are expressed as mean ± SE of three-five independent experiments from different DC donors. Mann-Whitney U-test was used and for pair-wise comparisons only. *p < 0.05, **p < 0.01, ***p < 0.001.
