Figure 2
The 3D spheroid-culture of human UC-MSCs significant upregulated ZC3H12A protein expression and induced profound changes in cytokine production compared with 2D UC-MSCs. (a) The expression of ZC3H12A and actin proteins in 2D- and 3D-cultured UC-MSCs was detected by Western blotting. The representative blots from same gel were cropped and showed in the picture. The Western Blotting experiments were performed twice to verify the result consistency. The full-length gels and blots are included in Supplementary Figure 2. The expression of ZC3H12A protein was significantly increased in 3D UC-MSCs compared with 2D UC-MSCs. Data are presented as means ± SE (n = 4); aP < 0.05 compared with 2D UC-MSCs. (b) The UC-MSCs cultures are as described in materials and methods. The culture medium was collected at 72 h time points, the concentrations of cytokines, as determined by multiplex-microbead immunoassay, are shown. Data are presented as means ± SE (n = 3); aP < 0.05 compared with 2D UC-MSCs. Abbreviations: ZC3H12A, the zinc finger CCCH-type containing 12A; HGF, hepatocyte growth factor; LIF, leukemia inhibitory factor; SCGF-β, stem cell growth factor-β; G-CSF, granulocyte-colony stimulating factor; IFN-α, interferon-α; M-CSF, macrophage-colony stimulating factor; SDF-1α, stromal cell-derived factor 1α; bFGF, basic fibroblast growth factor; MIF, macrophage migration inhibitory factor; SCF, stem cell factor; MCP-1, Monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor; UC-MSCs, umbilical cord-derived mesenchymal stem cells.
