Figure 6
From: Liver X Receptor exerts a protective effect against the oxidative stress in the peripheral nerve
LXR activation reduces ROS generation and cytotoxicity in vitro. MSC80 cells were pre-incubated with either TO9 or vehicle control for 72 h and then subjected to tert-butyl hydroperoxide (tBHP) treatment during 2 h. (a) Schematic time scale representation of the experiment performed. (b) Representative FACS analysis results for each condition (Vehicle control Ethanol: EtOH, Tertbutyl hydroperoxide: tBHP and TO9 + tBHP treatment) X axis represents DCFH-DA fluorescence and Y-Axis represents PI fluorescence. The scatter plot represents the entire population of cells with their respective DCFH-DA and PI fluorescence. The R1 gate considers only live cells to quantify DCFH-DA fluorescence and excludes dead cells. (c) Oxidative stress assessed by DCFH-DA fluorescence across 3 individual FACS experiments. (d) GSH assay results after tBHP treatment. Cell death assessed by PI fluorescence across 3 individual FACS experiments. Results are presented as the mean+/− SD values of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 by Tukey’s post hoc test after one-way ANOVA when compared with control.
