Figure 1
From: Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium
Comparison of palatal and buccal mucosa. Histological analysis of oral mucosa in 8-weeks-old mice. HE staining (A) and IHC staining for KRT10 (green) (B) or KRT14 (C) was performed using coronal sections of mouse head. Nuclei were counterstained with DAPI (blue). Boxes indicate the area shown at higher magnification in the right panels. E, epithelial tissue; M, Mesenchymal tissue; T, Tongue; B, bone. Note that KRT10 is highly expressed in keratinized mucosa, whereas KRT14 is expressed in basal cells of both keratinized and non-keratinized mucosa. (D) mRNA expression levels of Krt1 and Krt10 in palatal and buccal mocosa was measured by real time RT-PCR. The expression of each gene was normalized to that of S29 ribosomal RNA. Bars represent the mean values and standard deviation (+/−SD) (n = 3). ***p < 0.001 (Student’s t-tests). Results are representative data of at least three independent experiments. (E) TEM image of palatal mucosa (left) and buccal mucosa (right). Boxes indicate the area shown at higher magnification. Arrows indicate keratohyalin granules. C, stratum corneum; G, Granular layer; S, Superficial layer; I, Intermediate layer.
