Figure 4
From: Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium
Comparison of keratinized mucosa between new-born and aged WT and Col4a6-KO mice. (A,C) IHC staining for KRT10 (green) in palatal mucosa of WT and Col4a6-KO mice in new-born (A) and 28-week-old aged mice (D). Nuclei were counterstained with DAPI (blue). (E) Epithelial tissue; M, Mesenchymal tissue. The percentage of positive fluorescent signal for KRT10 in the area of palatal mucosa of new-born and aged mice is shown in graphs B and D, respectively. Bars represent the mean values and standard deviation (+/−SD) (n = 3). *p < 0.05 (Student’s t-tests). (E–F) IHC staining for KRT14 (green) in palatal mucosa of WT and Col4a6-KO mice in new-born (E) and 28-week-old aged mice (F). Note that KRT14 levels are higher in newborn WT mice compared to Col4a6-KO mice. In adult mice, the levels of KRT14 are identical in both WT and Col4a6-KO mice. Results are representative of at least three independent experiments.
