Figure 1
Brca1 knock-in mouse model for the RING and BRCT mutations. (a) Key knock-in mutations in domain structures of BRCA1 protein. Different colors are used to show the structures with RING domain in orange, nuclear export signal (NES) in red, the tandem nuclear localization signals (NLS) in green, the serine cluster domain (SCD) in purple, and two BRCT domains in blue. I26A: isoleucine to alanine at the 26 amino acid position. S1598F: serine to phenylalanine at the 1598 amino acid position. (b) Validation of different mutant mice by genotyping. Full-length gels of the PCR analysis are presented in Supplementary Fig. 1. (c) COBRA1 immunohistochemistry analysis in mammary gland of 8-week virgin mice. Representative results from at least 4 sets of animals. Scale bar = 50 µM.
