Figure 4
Palb2 and Cobra1 are efficiently deleted in mammary epithelium. (a) COBRA1 immunohistochemistry analysis in mammary gland of 8-week virgin mice. Representative results from at least 4 sets of animals. Scale bar = 50 µM. (b) Representative result of fluorescence-activated cell sorting of mouse mammary glands using cell surface markers EpCAM and CD49f. Cells are sorted to stromal, basal, and luminal populations. (c) mRNA analysis of Cobra1 and Palb2 using sorted stromal, basal and luminal cells. The numbers of animals used are: WT = 10, CKO = 4, PKO = 4, CKO/PKO = 5. Error bars represent s.e.m.
