Figure 2 | Scientific Reports

Figure 2

From: Rational cell culture optimization enhances experimental reproducibility in cancer cells

Figure 2

Glutamine and glutamate concentrations 48 hours after treatment with 0.01% DMSO ± 1 μM GLS1i using a prevalent assay method. A549 and H358 are known as sensitive and resistant cell lines, respectively, vis-à-vis glutaminase 1 inhibitors. Concentrations of (a) extracellular glutamine (b) intracellular glutamine and (c) intracellular glutamate measured by LC-UV after 24 hours of treatment with 0.010% DMSO ± 1.0 μM (final concentrations) GLS1i. For this single experiment, measurements were performed in triplicate for control and treated conditions. Cells had been seeded at a density of 8 × 105 cells/well in 1 ml of culture media 24 hours prior to commencing the experiment. Shown are the mean ± SEM for the 3 technical replicates per cell line and treatment condition. Unadjusted p-values of the differences between control and treated samples obtained using a two-tailed Student’s t-test are denoted with asterisks: **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Back to article page