Figure 6
From: Rational cell culture optimization enhances experimental reproducibility in cancer cells
Levels of various intracellular metabolites 24 hours after treatment with 0.01% DMSO ± 1 μM GLS1i using the improved culture conditions devised here. A549 and H358 are known as sensitive and resistant cell lines, respectively. Concentration of (a) glutamate and (b) glutamine measured by LC-UV, and relative abundance of the TCA cycle intermediates (measured by LC-MS) (c) alpha-ketoglutarate, (d) citrate and (e) fumarate in cells after treatment with or without 1 μM GLS1i. For each experiment, measurements were performed in triplicate for control and treated conditions. Shown are the mean ± SEM of 3 (A549 cell line; 9 data points per condition) or 2 (H358 cell line; 6 data points per condition) independent experiments. Unadjusted p-values of the differences between control and treated conditions obtained using a two-tailed Student’s t-test, the results of which are denoted with asterisks: **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
