Table 1 Predicted versus observed effects of treatment with the GLS1i inhibitor using the prevalent culture conditions compared with the optimized culture conditions devised in this study.
From: Rational cell culture optimization enhances experimental reproducibility in cancer cells
Predicted effect of GLS1i treatment… | Prevalent culture conditions | Optimized culture conditions |
|---|---|---|
…in sensitive A549 cells | ||
Cell numbers ↓ | ✖ | ✔ |
Glutamine consumption ↓ | ✔ | ✔ |
Glutamate production ↓ | ✖ | ✔ |
Glutamine (intracellular) ↑ | ✔ | ✔ |
Glutamate (intracellular) ↓ | ✖ | ✔ |
TCA cycle intermediates (intracellular) | ||
α-KG ↓ | ✖ | ✖ |
Citrate ↓ | ✖ | ✔ |
Fumarate ↓ | ✖ | ✔ |
….in resistant H358cells | ||
Cell numbers unchanged | ✔ | ✔ |
Glutamine consumption ↓ | ✔ | ✔ |
Glutamate production ↓ | ✖ | ✔ |
Glutamine (intracellular) ↑ | ✔ | ✔ |
Glutamate (intracellular) ↓ | ✔ | ✔ |
TCA cycle intermediates (intracellular) | ||
α-KG ↓ | ✔ | ✔ |
Citrate ↓ | ✖ | ✖ |
Fumarate ↓ | ✖ | ✔ |
