Figure 5
Gal-3 inhibition via a blocking antibody or gene knockdown increased CD66a expression in trophoblasts. (A) Trophoblasts (HTR-8 cells) were stimulated for 48 h with rhGal-3, P.g.-LPS, or a Gal-3 blocking antibody together with P.g.-LPS. CD66a mRNA expression was examined after exposure to P.g.-LPS and rhGal-3 with or without the Gal-3 antibody. (B,C) siRNA-mediated Gal-3 knockdown was performed for 48 h. CD66a expression at both the mRNA and protein levels. GAPDH or β-actin expression was detected as an internal control. The experiments were performed at least 3 times with similar results.
