Figure 3

Mitochondria in GCs and axonal processes show different correlations between axonal elongation and mitochondrial ATP or inner-membrane potential. Average (a) density, (b) mitochondrial ATP levels, (c) integrated mitochondrial ATP signals (density × mitochondrial ATP levels), and (d) TMRE intensity of growth cones (GC; blue), axonal processes near GCs (0–20 µm from GC; grey) and axonal processes relatively far from GCs (20–40 µm from GC; light grey). Correlations between the distance of axonal elongation with (e,h,k) mitochondrial ATP, (f,i,l) integrated mitochondrial ATP signal or (g,j,m) TMRE intensity at (e–g) GCs, (h–j) axonal processes near GCs and (k–m) axonal processes relatively far from GCs. Data were taken from (a–d) 29 neurons or (e–m) a subset of 18 neurons grown from within those 29 neurons. In (e–m), each color represents each neuron. Error bars represent SEM.