Figure 1 | Scientific Reports

Figure 1

From: Incorporation of a skeletal muscle-specific enhancer in the regulatory region of Igf1 upregulates IGF1 expression and induces skeletal muscle hypertrophy

Figure 1

Confirmation of the activity of candidate enhancers by luciferase assays. (a) A schematic of luciferase reporter vectors. The enhancer sequences are linked to the Igf1 mini-gene in both orientations. The yellow, grey, and red blocks represent the luciferase coding cassette, the IRES element, and the Igf1 mini-gene cassette, respectively. The blue arrows represent the enhancer sequences and indicate the orientation with respect to the Igf1 mini-gene. (b) The luciferase expression levels of the indicated constructs in Hepa1–6 cells (top), C2C12 myoblasts (middle), and C2C12 myotubes (bottom). The results are representative of three independent experiments. Bars represent the mean values, and the error bars represent the standard error of the mean (SEM). *P < 0.05 between indicated reporter vectors and the IGF1-basic construct; **P < 0.01 between indicated reporter vectors and the IGF1-basic construct. Statistical significances were analysed by Student’s t-test.

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