Figure 2 | Scientific Reports

Figure 2

From: Incorporation of a skeletal muscle-specific enhancer in the regulatory region of Igf1 upregulates IGF1 expression and induces skeletal muscle hypertrophy

Figure 2

Evaluation of sgRNAs targeting the three candidate insertion sites. (a) Schematic of the sgRNAs targeting the three candidate insertion sites. The protospacer-adjacent motifs (PAM) are labelled in red. The distance between each candidate insertion site and the transcriptional start site is shown. (b) T7EN1 assay detection of the efficiency of Cas9-mediated cleavage at the indicated targets. The blue triangles indicate the locations of the PCR products before T7EN1 digestion. The asterisks indicate cleaved PCR amplicon fragments of the expected sizes after T7EN1 digestion. The extra blots in lanes of the sgRNAs targeting the site 2 have no relation to the gene editing events and may arise from the SNPs existing in the C2C12 genome. The sgRNAs Igf1–1, Igf1–9 and Igf1–7 were used to generate double-stranded breaks to stimulate homologous recombination in each of the three respective candidate sites. NC: WT C2C12.

Back to article page