Table 1 Generation of a genetically modified mouse model.

From: Incorporation of a skeletal muscle-specific enhancer in the regulatory region of Igf1 upregulates IGF1 expression and induces skeletal muscle hypertrophy

Experiment times

Number of Injected zygotes

Number of recipients

Number of pregnant recipients

Number of newborns

Pregnancy rate

Live-birth rate

Number of the positives

F0 positive Litters

Positive rate

1

250

10

4

13 (5♂ + 8♀)

40.00%

5.20%

3 (1♂ + 2♀)

1#♂, 6#♀, 10#♀

23.07%

2

160

8

3

19 (11♂ + 8♀)

37.50%

11.88%

9 (5♂ + 4♀)

17#♂, 18#♂, 19#♂, 21#♂, 22#♀, 26#♀, 27#♀, 29#♂, 31#♀

47.37%

Total

410

18

7

32 (16♂ + 16♀)

38.89%

7.80%

12 (6♂ + 6♀)

 

37.50%

  1. 5 ng/µl Cas9 mRNA, 2.5 ng/µl sgRNAs and 5 ng/µl donor vector targeting the site 2 locus were co-injected into zygotes of the C57BL/6 mouse. To circumvent random integrations, a circular donor vector was used instead of a linear one. The sex distributions of the total number of newborns and positive newborns are shown in parentheses respectively. The F0 positive mice used to produce offspring are labelled in bold.
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