Figure 2

Histological analysis of spheroids. (A–D) Ultrastructures of cerebellum of 5- to 9-months-old mice were analyzed via transmission electron microscopy. (A) A representative image of normal axons of Elavl3^+/+ Purkinje cells. (B–D) Representative images of axonal spheroids detected in Elavl3^−/− Purkinje cell. (B) The boundary that just enters from the axon to the spheroid. (C and D) Subcellular organelles such as mitochondria (C and D), multiple membranous bodies (C), and smooth endoplasmic reticulum (D) are observed in spheroids. Note that spheroids are surrounded by myelin sheaths. Scale bar, 1 μm. (E–G) The cerebellar sections of Elavl3^+/+ and Elavl3^−/− mice at 9 months of age were immunostained with the indicated antibodies. Spheroids were surrounded by myelin sheaths (E) and filled with neurofilament-H (NF-H; F) and amyloid precursor protein (APP; G). Scale bar, 10 μm.