Figure 4

Expression analysis of KIF3A and KIF3C. (A) RT-PCR analysis of KIF3A and KIF3C in the cerebella of 2-month-old Elavl3^+/+ and Elavl3^−/− mice. Expression levels of KIF3A and KIF3C mRNA were significantly decreased in Elavl3^−/− cerebella. (n = 6 mice for each group). **P < 0.01 (Student’s t-test). Full-length gel is presented in Supplementary Fig. S5. (B) The cerebellar sections of Elavl3^+/+ and Elavl3^−/− mice at 2 or 9 months of age were immunostained with the indicated antibodies. (C) The fluorescent signal ratios of KIF3A and KIF3C in the cytosolic region of Purkinje cells were quantified (also see the Methods). The fluorescent signal ratios of KIF3A and KIF3C were significantly decreased in Elavl3^−/− Purkinje cells in both 2- and 9-month-old mice (n = 316, 288, 298, and 269 Purkinje cells from 2 independent mice for KIF3A, and n = 264, 243, 217, and 262 Purkinje cells from 2 independent mice for KIF3C). Scale bar, 10 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA followed by the Bonferroni post hoc test).