Figure 2

Relative expression levels of the miRNAs with different representations found in the plasma of the AIS patients versus the control healthy subjects. Box plot of the relative expression levels of the miRNAs analysed by RT-qPCR, normalised to miR-191 as an endogenous control and calculated using the 2^-∆∆Ct method. (a) miR-122 (Fold Change, FC > 2.65; p < 0.05; (b) miR-27a (FC > 1.87; p < 0.005); (c) miR-223 (FC > 1.5; p < 0.0001); (d) miR-1306 (FC > 1.03; p = 0.38); and (e) miR-671 (FC > 0.81; p = 0.53). Samples were ordered according to their corresponding group: controls (C) or AIS patients (P). An independent samples t-test was applied to analyse the biospecimens from the 17 healthy subjects (6 males and 11 females) and the 29 AIS patients (5 males and 24 females). A p < 0.05 was considered to indicate a significant difference.