Figure 4

Number of the sequence tags representing the gene expression information. (A) Five genes at varying average expression levels at bulk were selected and the number of the sequence tags representing their expression levels (y-axis) at the indicated sequence depth (x-axis) is shown. Sequence tags per cell were serially diluted computationally starting from the micro-chamber datasets. Error bars correspond to the divergence between individual cells. Since, the cells having zero tags are not represented explicitly, those populations are separately indicated in the table in the margin. Cellular divergences of the number of the sequence tags are also represented in the most bottom table. (B) Results of the similar analysis are shown in separately for individual genes at the average sequencing depth of 5,000 tags per cell.