Figure 6
From: Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
The Intestine Chip resembles human native duodenum. A curated heatmap was generated for genes selected through z-score comparison and template matching method showing genes with similar expression profiles in the mechanically active Intestine Chip (with fluid flow at 60 μL h−1 and cyclic stretching at 10%, 0.2 Hz; Intestine Chip) and in vivo duodenum in comparison to in vivo jejunum and ileum, as well as 3D organoids and Caco-2 based Gut Chip (Caco-2 Gut Chip) and static Caco-2 Transwell models. 3D organoids and Intestine Chip were established from the same duodenal tissue biopsies derived from 3 healthy donors and differentiated for 4 days in DM medium before assessment. Gene expression data for normal human small intestine (duodenum, jejunum, and ileum), Caco-2 Gut Chip, and Caco-2 Transwell were obtained from Gene Expression Omnibus (GEO) database55,57. Selected genes shown belong to the following GO terms: Defense Response (GO:0006952), Drug Transport (GO:0015893), Digestive System Process (GO:0022600), Regulation of Epithelial Cell Proliferation (GO:0050678), and Response to Nutrients (GO:0007584). See Supplementary Table S1 for full names of all genes and GO categories.
