Table 1 The protein yield of CTendo45 and mutants after Ni2+ affinity chromatographic purification. aValues are the means ± SD of three replicates.

From: Engineering the conserved and noncatalytic residues of a thermostable β-1,4-endoglucanase to improve specific activity and thermostability

Enzyme

Protein yield (mg/L)a

CTendo45

358.9 ± 46.1

R29H

256.3 ± 27.5

Y30F

323.0 ± 55.1

W31S

344.6 ± 49.2

Y173F

407.8 ± 37.6

Y30F/Y173F

321.4 ± 39.7

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