Table 2 Activities of CTendo45 and mutants on CMC-Na and β-D-glucan.

From: Engineering the conserved and noncatalytic residues of a thermostable β-1,4-endoglucanase to improve specific activity and thermostability

Substrate

Enzyme

Activity(IU/mg)a

Fold

CMC-Na

CTendo45

1.21 ± 0.08

1.00

R29H

0.21 ± 0.03

0.17

Y30F

1.63 ± 0.10

1.35

W31S

0.44 ± 0.01

0.36

Y173F

2.26 ± 0.04

1.87

Y30F/Y173F

0.83 ± 0.05

0.68

β-D-glucan

CTendo45

2.07 ± 0.14

1.00

R29H

0.46 ± 0.03

0.22

Y30F

2.50 ± 0.13

1.21

W31S

0.78 ± 0.04

0.38

Y173F

3.46 ± 0.21

1.67

Y30F/Y173F

0.94 ± 0.07

0.45

  1. Each reaction was performed in 50 mM acetate buffer (pH 4) and incubation at 60 °C for 30 min using substrates of 1% CMC-Na and 0.2% β-D-glucan, respectively. aValues are the means ± SD of three replicates.
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