Table 3 Half-life of CTendo45 and mutants in thermal inactivation.

From: Engineering the conserved and noncatalytic residues of a thermostable β-1,4-endoglucanase to improve specific activity and thermostability

Substrate

Enzyme

t1/2 and residual relative activity

80 °C

90 °C

CMC-Na

CTendo45

150 min (48.8%)

35 min (52.9%)

Y30F

120 min (46.2%)

20 min (54.1%)

Y173F

210 min (48.9%)

50 min (53.0%)

Y30F/Y173F

240 min (51.8%)

60 min (49.5%)

β-D-glucan

CTendo45

130 min (52.6%)

28 min (49.8%)

Y30F

100 min (46.7%)

15 min (54.4%)

Y173F

180 min (53.7%)

35 min (43.1%)

Y30F/Y173F

220 min (48.4%)

45 min (46.5%)

  1. Half-life (t1/2) was defined as the time that the enzyme activity declined to half of the initial activity value at temperatures of 80 °C and 90°, respectively. Untreated enzymes are considered as controls (100%). The residual relative activities are shown in brackets.
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