Figure 3

In vitro characterization (a) Competition binding assay (apparent IC₅₀) of [Lys³⁷(DTPA)]GIP_1-42 on NES2Y cells. [Lys³⁷(¹¹¹In-DTPA)]N-acetyl-GIP_1-42 was used as a radiotracer (b) binding and internalization kinetics of ¹¹¹In-GIP in BHK-GIPR positive cells and NES2Y cells. Cell bound and internalized fractions are corrected for non-specific binding and accumulation, as determined by co-incubation with an excess unlabeled GIP_1-42.