Figure 3
Increased thrombus resolution in the Tbx21−/− mice. (A) Thrombus sizes determined by high frequency ultrasound (HFSU) calculated as percentage of the size at d2 in C57BL/6 and Tbx21−/− group at indicated time points following IVC stenosis. Mean ± SEM; two-way ANOVA/Bonferroni post hoc test; n = 7; *p ≤ 0.05; ***p ≤ 0.001. (B) Flow cytometric identification of thrombus inflammatory cells at indicated days after subtotal ligation of control compared to Tbx21−/− mice. Data shown as median with interquartile range and mean ± SEM; t-test; n = 4–5; *p ≤ 0.05. (C) Left panel: Histological staining (MTC staining) of IVCs after IVC stenosis at indicated time points, representative pictures are shown. Magnification x10. Right panel: Summary of the quantitative analysis on fibrin and collagen contents of venous thrombi in 4 mice per group for MTC staining. Mean ± SEM; t-test; n = 4–5; ****p ≤ 0.0001.
