Table 2 Comet assay results of coelomocytes in E. fetida exposed to tribenuron-methyl, tebuconazole and the mixture of both pesticides (mean ± SD). The same letters in the columns indicate no significant difference between treatments at the same time (p < 0.05).
Pesticide | Dose (mg kg−1) | Length of Comet (μm) | Olive Tail Moment (OTM) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
3 | 7 | 14 | 21 | 28 d | 3 | 7 | 14 | 21 | 28 d | ||
Tribenuron-methyl | 0 | 3.04 ± 0.73a | 3.21 ± 1.02a | 2.98 ± 0.85a | 3.64 ± 0.92a | 3.33 ± 1.14a | 0.74 ± 0.20a | 0.56 ± 0.14a | 0.44 ± 0.20a | 0.46 ± 0.17a | 0.65 ± 0.23a |
0.025LC50 | 3.32 ± 0.2a | 2.54 ± 0.65a | 3.3 ± 0.92a | 2.76 ± 0.84a | 3.5 ± 1.23a | 0.65 ± 0.17a | 0.54 ± 0.15a | 0.39 ± 0.12a | 0.72 ± 0.22a | 0.55 ± 0.15a | |
0.05LC50 | 2.78 ± 0.8a | 3.03 ± 0.85a | 2.88 ± 0.64a | 3.54 ± 1.04a | 3.2 ± 0.8a | 0.71 ± 0.28a | 0.62 ± 0.16a | 0.58 ± 0.17a | 0.55 ± 0.15a | 0.62 ± 0.18a | |
0.1LC50 | 3.54 ± 1.13a | 3.05 ± 0.76a | 3.3 ± 0.95a | 3.2 ± 0.8a | 3.23 ± 0.68a | 0.59 ± 0.14a | 0.75 ± 0.20a | 0.53 ± 0.15a | 0.62 ± 0.18a | 0.53 ± 0.12a | |
Tebuconazole | 0 | 2.54 ± 0.85a | 2.65 ± 0.85a | 3.05 ± 0.54a | 2.85 ± 0.81a | 3.1 ± 0.88a | 0.72 ± 0.15a | 0.67 ± 0.20a | 0.58 ± 0.23a | 0.64 ± 0.22a | 0.56 ± 0.16a |
0.025LC50 | 2.88 ± 0.6a | 3.04 ± 0.80a | 2.89 ± 1.13a | 3.24 ± 0.68a | 2.86 ± 0.64a | 0.68 ± 0.16a | 0.53 ± 0.12a | 0.70 ± 0.15a | 0.58 ± 0.12a | 0.63 ± 0.14a | |
0.05LC50 | 2.83 ± 0.76a | 2.98 ± 0.56a | 3.32 ± 0.78a | 3.07 ± 0.78a | 3.22 ± 0.59a | 0.53 ± 0.17a | 0.61 ± 0.13a | 0.58 ± 0.14a | 0.63 ± 0.10a | 0.65 ± 0.10a | |
0.1LC50 | 3.18 ± 0.75a | 3.34 ± 0.95a | 3.24 ± 0.74a | 3.35 ± 0.90a | 3.05 ± 0.0.57a | 0.78 ± 0.22a | 0.70 ± 0.16a | 0.65 ± 0.12a | 0.59 ± 0.13a | 0.67 ± 0.12a | |
Tribenuron-methyl + Tebuconazol(1:1 toxicity) | 0 | 3.23 ± 0.75a | 2.79 ± 0.77a | 3.04 ± 0.74a | 2.96 ± 0.57a | 3.20 ± 0.84a | 0.64 ± 0.13a | 0.70 ± 0.12a | 0.68 ± 0.22a | 0.64 ± 0.13a | 0.67 ± 0.14a |
0.025LC50 | 3.05 ± 0.73a | 3.06 ± 0.82a | 2.79 ± 0.57a | 3.21 ± 0.75a | 3.24 ± 0.94a | 0.72 ± 0.15a | 0.62 ± 0.14a | 0.74 ± 0.15a | 0.62 ± 0.12a | 0.75 ± 0.15a | |
0.05LC50 | 3.23 ± 0.73a | 3.22 ± 0.68a | 3.12 ± 0.68a | 3.08 ± 0.85a | 3.04 ± 0.73a | 0.65 ± 0.14a | 0.82 ± 0.21a | 0.66 ± 0.15a | 0.70 ± 0.15a | 0.85 ± 0.16a | |
0.1LC50 | 3.44 ± 0.73a | 3.47 ± 0.94a | 2.89 ± 0.58a | 3.16 ± 0.83a | 3.25 ± 1.04a | 0.84 ± 0.23a | 0.91 ± 0.22a | 0.74 ± 0.14a | 0.73 ± 0.21a | 0.69 ± 0.12a |