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Figure 1

From: The Fate of a Hapten - From the Skin to Modification of Macrophage Migration Inhibitory Factor (MIF) in Lymph Nodes

Figure 1

Laser scanning confocal microscopy (LSCM) images of cryosectioned tissues. Mice were exposed to 25 µl of tetramethylrhodamine isothiocyanate (TRITC) (11 mM) (a,c) or the non-sensitizing structural analogue tetraethylrhodamine (Rhodamine B) (11 mM) (b) on the dorsal side of each ear for three consecutive days. Eighteen hours after the last exposure, the mice were sacrificed and ears (a,b) and draining lymph nodes (c) were excised, snap frozen and sectioned (10 µm and 14 µm, respectively). Slides were fixed in acetone. To be able to detect Rhodamine B, the laser intensity was increased 20 times compared to the settings for TRITC. The scale bars refer to 75 µm (a,b) and 500 µm (c). h, hair follicle; ca, cartilage; e, epidermis; sc, stratum corneum. The structures of TRITC and Rhodamine B are shown at the top of the figure.

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