Figure 2

Flow cytometry analysis of lymph node cell populations. Cells from ear draining lymph nodes isolated from mice exposed to TRITC (5.6 mM in acetone:dibutyl phthalate, 1:1, 25 µl) (a–d) or vehicle (acetone:dibutyl phthalate, 1:1, 25 µl) (e–h) on both ears for three consecutive days were analyzed by flow cytometry. Lymph node cells from each treatment group were pooled before analysis. The dot plots show unstained cells (a,e) and cells stained with allophycocyanin (APC) labeled antibodies toward T cells (CD3ε; b,f), B cells (CD19; c,g), or DC (CD11c; d,h). Q1 (upper left quadrant) shows the proportion of APC positive/TRITC negative cells and Q2 (upper right) shows the proportion of double positive cells. Q3 (lower left) shows the proportion of double negative cells and Q4 (lower right) the proportion of APC negative/TRITC positive cells.