Figure 6 | Scientific Reports

Figure 6

From: PREP1 tumor suppressor protects the late-replicating DNA by controlling its replication timing and symmetry

Figure 6

PREP1 down-regulation causes loss of Lamin B1. (A) Immunofluorescence staining of Lamin B1 (green) and PREP1 (red) in HeLa cells transfected with control siLuc (top) or siPREP1 oligonucleotides (bottom). The upper right panel shows the quantitation of PREP1 and Lamin B1 signal intensity per cell in control siLuc (black) and siPREP1-transfected cells (red). (B) Immunoblotting analysis on total cell lysates of HeLa cells treated with control (Luc) or PREP1 siRNA, as indicated. (C) qPCR analysis of PREP1 and Lamin B1 mRNA in siLuc and siPREP1 cells (average ± SD of three replicates).

Back to article page