Figure 3
Mitochondria of H3S28A mutants show a generally reduced oxygen consumption with increased release of ATP and unchanged H2O2 production. (a) Oxygen consumption rate under basal complex-I-respiration and after stimulation with 1 mM ADP (left) and ratio of both states (right). Results are normalized to maximal uncoupled respiration via 30 mM FCCP (nH3S28S, nH3S28A = 11 samples à 20 flies, 7–10 days old). (b) Production of ATP (left) and ROS (right) immediately after quantification of ADP-stimulated respiration (nH3S28S, nH3S28A = 7 [ATP]/8 [ROS] samples à 20 flies, 7–10 days old).
