Table 1 Targeted enzymes detected in gel group 2, 4, 7, 8 and 9 following sampling of the cultures at 24 h (the beginning of lipid accumulation).

2

FAS

S2JBT1

4

10.71

9.05

10.16

12.83

12.95

9.05

81.5

66.8

70.1

65.5

70.0

68.6

APTGLDQSR

DIAVLK

TFNDTALR

YVVDSIAR

LAIVDR

VVVTTSR

2

64.67

ME

S2J9V8

2

14.3611

78

10.58

11.51

9.45

77.5

74.3

61.2

76.4

73.4

AELPELCQTIR

ILGLGDLGTNGIGIPIGK

TLPIILDLGTNNEK

QIQSYFQIEHNMTEEEAK

HVFWIVDSK

10

57.68

ACL

S2JM91

7

14.49

10.85

10.11

18.22

17.38

8.24

9.07

18.33

14.11

14.54

17.09

20.35

13.23

17.61

11.02

10.27

8.56

89.1

75.4

64.7

89.9

89.5

67.3

66.1

88.5

80.0

76.4

95.1

91.5

75.1

90.6

73.5

76.6

68.1

LVAKPDQLIK

HGLLTLNK

QWIEER

IDQTAEFEAGPK

EEAYIQELDGK

TILDLMTR

ALTEFK

GGPNYQEGLR

AFVYGMQPR

FYWGTK

TIAIIAEGVPER

TTDGVYEGVAIGGDR

KPASFVSTIVDDR

AYDSGMTPR

EFVTSMR

LIPGIGHK

VEEITTSK

15

246.91

ACC

S2J3C8

1

13.36

11.78

9.34

10.58

75.7

74.3

76.3

76.6

LSEIESR

TADYLISESDR

ADEDMDDTAFR

LGADPIDR

2

57.68

PC

S2JTP7

5

11.08

14.87

12.38

8.07

9.68

10.88

9.74

10.89

12.58

10.61

65.6

73.2

82.4

62.0

61.5

75.3

76.5

76.9

75.4

70.6

TAHELSMR

TVAVFSHEDR

HIEVQLLADR

NAGTAEFLVDNQNR

VTTEDPELNFQPDTGK

GFAIQCR

ALVEFR

TNIPFLQR

DAHQSLLATR

LGIDAVK

6

110.78

MDH

S2J7L6

3

10.77

14.38

13.17

15.21

14.91

12.02

12.83

80.0

88.7

84.3

82.9

84.9

62.5

72.7

KPGMTR

INAGIVR

DLAVAAAK

IFGVTTLDIVR

IQFGGDEVVK

DGAGSATLSMAYAGAR

GSTFITEGAK

38

92.39

40 S Ribosomal protein S0

S2JWG6

2

10.41

8.45

7.25

72.6

71.7

61.5

ADGINLINIGK

MTPYIHK

HAIGLIYWLLAR

10

26.11

60 S Ribosomal protein L6

S2JHQ8

2

13.42

7.23

11.58

69.9

62.1

71.4

VNQAYVIATSTK

QTAVAAPAK

GQFPHNMK

12

32.23

4

PFK

S2K2L9

2

12.39

12.06

11.53

10.81

80.6

75.1

77.4

70.9

IGVLTSGGDAPGMNPCVR

GLFTPQAQATLAR

AVELTHEVAK

TVEFYK

5

46.70

7

ACL

S2JM91

4

12.76

9.76

9.96

11.16

12.23

10.17

61.3

64.4

75.4

64.3

74.5

61.2

EGDYILFTHEGGIEVGDVDAK

IDQTAEFEAGPK

TILDLMTR

TIAIIAEGVPER

TTDGVYEGVAIGGDR

LIPGIGHK

7

66.04

ACC

S2J3C8

1

12.55

13.07

13.52

76.4

74.3

75.7

IYLSANSGAR

ENYQEDETIR

LSNFDIKPCFIENR

2

39.14

PC

S2JTP7

2

11.52

15.09

12.98

14.52

10.47

65.6

73.2

82.4

75.4

76.9

TAHELSMR

IGQYTPVAAYLAQDEVVR

SEIDIPVLR

YFLSKPEINEEFHVEIEEGK

NSALEVVTR

5

64.58

PK

S2J681

2

13.21

12.37

11.05

78.4

76.5

66.7

TGLMLNDTEVPIK

WDEDVESR

WGIQQGK

5

36.63

8

ME

S2J9V8

2

11.52

11.78

10.59

14.36

76.4

74.3

61.2

77.5

SGYLNEGK

AELPELCQTIR

GDNEGQQYK

ALADSLTEEEISK

7

48.52

G-6-PDH

S2KGV4

3

12.54

11.26

9.17

79.4

71.5

69.8

TYPALFGLYR

NVMNLR

FANVLFGHAWDR

6

32.97

NADP⁺: ICDH

I1CKX9

2

13.22

11.68

9.79

78.3

76.9

66.8

LVPGWTNPIIIGR

LIDDMVAQALK

MPLYLSTK

8

34.69

9

MDH

S2J7L6

2

12.91

11.58

12.14

78.5

69.7

73.2

LSQEQIEK

FALNIVEAVVAGK

LFSAATSELK

9

36.63

6-PGDH

S2KIR3

2

14.71

13.22

10.04

9.58

81.8

76.2

75.9

69.1

MVHNGIEYGDMQLICEIYQVMK

WTGIDSLDR

GILFVGSGVSGGEEGAR

ILDTAGQK

12

47.55