Table 3 Targeted enzymes detected in gel group 2, 7, 8 and 9 following sampling of the cultures at 96 (after cessation of lipid accumulation phase).
Gel Group | Protein | Accession number | Distinct peptide | Peptide score | SPI (%) | Peptide sequence | Peptide coverage (%) | Protein score |
|---|---|---|---|---|---|---|---|---|
2 | FAS | S2JBT1 | 2 | 10.71 12.83 12.95 13.86 | 81.5 67.5 70.9 88.3 | IEGALLIAITMEPAAR EFGQALIENCVDVLAESPVYK IMIAGATEDITEESSYEFANMK STLQNEILGDLQK | 2 | 50.35 |
7 | ACL | S2JM91 | 4 | 12.76 12.23 11.16 10.21 9.96 9.76 | 61.3 74.5 64.3 61.2 75.4 64.4 | EGDYILFTHEGGIEVGDVDAK TTDGVYEGVAIGGDR TIAIIAEGVPER EGDYILFTHEGGIEVGDVDAK TILDLMTR TIAIIAEGVPEK | 8 | 66.08 |
| Â | ACC | S2J3C8 | 1 | 10.58 9.35 11.78 13.36 | 76.4 77.8 74.3 75.7 | ANAEYIR DEVQATR VEVYQEVK IYLSANSGAR | 1 | 40.07 |
| Â | PC | S2JTP7 | 3 | 11.08 9.74 10.51 10.83 12.31 12.47 14.86 | 65.6 77.5 70.6 76.9 82.4 75.5 73.2 | TAHELSMR DCSVQR HYFIEINPR LVQFQNR SEIDIPVLR LLYSCFEANVR METVVTSPVAGK | 7 | 81.81 |
8 | ME | S2J9V8 | 2 | 11.58 11.93 12.73 14.52 | 76.4 77.5 61.2 74.3 | YILMAQLR AELPELCQTIR NYRPTDTEGFEPEIAVISDGK IVFYGAGSAAIGVAR | 6 | 47.73 |
9 | MDH | Â | 2 | 9.98 15.21 11.18 13.03 12.83 | 88.7 82.9 84.9 62.5 72.7 | DLAVAAAK QFNVYDPR IQFGGDEVVK DGAGSATLSMAYAGAR GSTFITEGAK | 18 | 62.23 |
