Table 1 Oligonucleotides used in this study.

From: RACK1 is indispensable for porcine reproductive and respiratory syndrome virus replication and NF-κB activation in Marc-145 cells

Name

Sequence 5′—3′)

RACK1_F_seq

CGCCACCATGACTGAGCAGAT

RACK1_R_seq

CTAGCGCGTGCCGATGGT

siRACK1_1 (sense)

ccaucaagcuauggaauacdTdT

siRACK1_1 (antisense)

guauuccauagcuugauggdTdT

siRACK1_2 (sense)

caaauacacuguccaggaudTdT

siRACK1_2 (antisense)

auccuggacaguguauuugdTdT

siScramble (sense)

uucuccgaacgugucacgudTdT

siScramble (antisense)

acgugacacguucggagaadTdT

RACK1_F

TTCTCCTCTGACAACCGGCA

RACK1_R

GCCATCCTTGCCTCCAGAA

Viral N_F

AATGGCCAGCCAGTCAATCA

Viral N_R

TCATGCTGAGGGTGATGCTG

IFNα_F

gtgctcagctgcaagtcaag

IFNα_R

tggtttgagccttttggaac

GAPDH_F

TGGAAAAACCTGCCAAGTACG

GAPDH_R

ATGAGGTCCACCACCCTGTT

  1. CGCCACC, as the conserved sequence of Kozak sequence, is tagged to the 5′ of the start codon of the forward primer sequence to amplify the full sequence of the RACK1 mRNA.
Back to article page