Figure 1
Alternative splicing of ALCAM (ALCAM-Iso2) promotes metastatic dissemination. (a) Schematic describing ALCAM splice isoforms 1 and 2. (b) Immunoblot of parental HT1080 cells (Par) and HT1080 cells stably overexpressing ALCAM-Iso1 (Iso1-OE) or ALCAM-Iso2 (Iso2-OE); ICD: intracellular domain. (c) Schematic describing avian embryo spontaneous metastasis assay. (d) Representative images of gross (tumor is outlined in dashed line) HT1080 xenografts and metastasis to the distal chorioallantoic membrane (CAM), indicated by bioluminescence (Biolum.). (e) Wet weight of HT1080 xenografts removed from CAM of chicken embryos. Three independent experiments were combined for the analysis (N = 23, 18, 25, respectively). (f) Quantification of metastasis. Metastasis was measured by luciferase activity in a 1.5 cm core of distal CAM. Two cores per embryo from at least 10 embryos were measured. Relative bioluminescence (RL) represents mean value of two CAM sections per chick and was calculated using the integrated density tool (IntDen, 1 × 106) in ImageJ. (e,f) P-values were calculated using Kruskal-Wallis test with Dunn’s post-test, ns: not significant, *P < 0.05. Graphs display mean with 95% confidence interval. Medians are reported.
