Figure 2

Growth of CTNS mutants defective in lysosomal targeting. The S. cerevisiae met15Δ yct1Δ strain (ABC 1580) was transformed with wild-type CTNS and the mutant of defective in lysosomal trafficking (CTNS-Δgydql) expressed under TEF promoter, along with, Y363A and L366A mutants, and vector control, examined by serial dilution spotting on minimal media containing 200 µM Methionine & 500 µM L-cystine. The photographs were taken after 2 days of incubation at 30 °C. The experiment was repeated with two independent transformations.