Figure 5

Functional analysis of CTNS and CTNS-Δgydql transformed in different vps mutant backgrounds. Different vacuolar protein sorting defective mutants (met15Δ:BY4741) transformed with (a) CTNS-Δgydql and (b) CTNS expressed under TEF promoter, and examined by dilution spotting on minimal media containing 200 µM Methionine, & 500 µM L-cystine expressed as described in experimental procedures. The photographs were taken after 3 days of incubation at 30 °C. The experiment was repeated with three independent transformations.